Equal adhesion to pneumocytes by pneumococci inducing bacteraemia and pneumonia

Authors

  • Ying Qu Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China
  • Meng Li Department of Laboratory Medicine, The first Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Province, China
  • Chunyan Gao Department of Laboratory Medicine, Tangshan Maternal and Child Health Care Hospital, Tangshan, Hebei Province, China
  • Jin Zhang Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China
  • Xinhua Luo Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China
  • Guizhen Wang Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China
  • Xinyu Jiang Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
  • Jinhong Yang Department of Laboratory Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China
  • Xiangyang Li Department of Laboratory Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China
  • Dakang Hu Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China
  • Wushuang Zhu Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang Province, China
  • Weiwei Shen Department of Microbiology, Taizhou City center for Disease Control and Prevention, Taizhou, Zhejiang Province, China

DOI:

https://doi.org/10.47391/JPMA.11-784

Abstract

Abstract

Streptococcus pneumoniae (S. pneumoniae) is a leading agent worldwide, which could cause community-acquired pneumonia, bacteraemia, and meningitis. However, the pathogeneses remain unclear. This study was conducted to investigate gene pneumococcal surface antigen A (psaA) expression and the adhesion differences of various S. pneumoniae strains. A total of 24 (N) S. pneumoniae strains were collected: 11 from blood (bd-SP), 12 from sputum (sd-SP) and one was ATCC49619. One millilitre of A549 pneumocytes (3.3×108/L) and 100 µl of each S. pneumoniae strain at 1.0 McFarland were mixed and incubated under 37 °C and 5% CO2 for three hours. The cells were centrifuged and extracted for psaA mRNA analysis. The former experiment was redone. After culture, the adherent cells were collected and cultured on blood agar plates. The ?CT values of psaA were 18.9, 29.9 (±2.5), 29.6 (±2.0) and 16.0, 17.0(±3.3), 18.6(±3.8) for ATCC49619, bd-SP and sd-SP before and after stimulation respectively, with the colony units of 23, 68.4 (±6.7) and 59.1 (±7.7), which showed equal adhesion between bd-SP and sd-SP. Moderate psaA expression and adhesion of S. pneumoniae might facilitate its pathogenesis, excess of which induces faster S. pneumoniae clearance.

Key Words: Streptococcus pneumoniae; Alveolar epithelial cells; Virulence; Adhesion; pneumonia.

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Published

2022-05-07

How to Cite

Ying Qu, Meng Li, Chunyan Gao, Jin Zhang, Xinhua Luo, Guizhen Wang, Xinyu Jiang, Jinhong Yang, Xiangyang Li, Dakang Hu, Wushuang Zhu, & Weiwei Shen. (2022). Equal adhesion to pneumocytes by pneumococci inducing bacteraemia and pneumonia. Journal of the Pakistan Medical Association, 72(01), 146. https://doi.org/10.47391/JPMA.11-784

Issue

Vol. 72 No. 01 (2022): JANUARY

Section

RESEARCH ARTICLE