Seroprevalence of toxoplasmosis in humans and animals in Pakistan: a systematic review and metadata analysis Authors Sarfraz Ur Rahman Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan Aqib Saeed Institute of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan Sakandar Khan Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan Zia Ul Rehman Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan Muhammad Imran Rashid Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan Haroon Akbar Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan DOI: https://doi.org/10.47391/JPMA.5699 Keywords: Livestock, Humans, Random-effect model, Toxoplasma gondii, Seroprevalence Abstract Objective: To review the seroprevalence of toxoplasmosis in Pakistan. Method: The systematic review comprised search on Science Direct, Google Scholar, PubMed and Scopus databases for studies related to the seroprevalence of toxoplasmosis in Pakistan published between 2006 and 2020 which used serological diagnostic tests to detect Toxoplasma gondii. Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were used throughout the review and statistical analysis was done using forest plot and random effect model. Results: Of the 7093 human studies initially found, 20(0.28%) were reviewed. Of the 16,432 animal studies, 16(0.09%) were selected for detailed review. The pooled seroprevalence of toxoplasmosis in humans, calculated in this review was found as 76 (%) (95% confidence interval: 69-83%). Seroprevalence of human toxoplasmosis was higher in Khyber Pakhtunkhwa (31.7 (%)) than Punjab (20.4 (%)). Pooled seroprevalence in animals calculated in this review was found as 69 (%) (95% confidence interval: 64-74%). Seroprevalence in animals was higher in Khyber Pakhtunkhwa (44.7 (%)) than Punjab (29.4%). Conclusion: The seroprevalence of toxoplasmosis in both humans and animals should be studied it other parts of Pakistan as well. Key Words: Livestock, Humans, Random-effect model, Toxoplasma gondii, Seroprevalence. Downloads Full Text Article Published 2023-04-15 How to Cite Sarfraz Ur Rahman, Aqib Saeed, Sakandar Khan, Zia Ul Rehman, Muhammad Imran Rashid, & Akbar, H. (2023). Seroprevalence of toxoplasmosis in humans and animals in Pakistan: a systematic review and metadata analysis. Journal of the Pakistan Medical Association, 73(05), 1061–1068. https://doi.org/10.47391/JPMA.5699 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 73 No. 05 (2023): MAY Section Systematic Reviews License Copyright (c) 2023 Journal of the Pakistan Medical Association This work is licensed under a Creative Commons Attribution 4.0 International License.