Molecular identification of Hypomyces chrysospermus mycoparasitic fungus isolated from mushrooms in a local market of Iraq

Abstract

In this study, mycoparasitic fungus was identified by
using the rDNA internal transcribed spacer barcode
marker, i.e. ITS rDNA gene. Amplicons were sequenced
and identified by NCBI – BLAST (Basic local alignment
search tool). The BLAST results revealed that NOOR strain
matched 100% with accession numbers [MH854754.1].
The phylogenetic tree shows close relationship between
NOOR strain and H. chrysospermus [MH854754-1], H.
chrysospermus [MZ389105-1], H. chrysospermus [MK605327-
1], H. chrysospermus [MT595227-1], H. [EU816370-1], H.
chrysospermus [MG685885-1].
The Iraqi isolate was recorded as NOOR strain in the
National Centre for Biotechnology Information for the
first time in Iraq.
Keywords: Hypomyces chrysospermus, Phylogeny, DNA,
Ribosomal, Biotechnology