The hepatoprotective potentials of Olea europaea L. leaves against carbon tetrachloride-induced hepatic injury in rats Authors Amber Ayaz Memon Department of Physiology, University of Karachi, Karachi, Pakistan. Lubna Naz different mechanism, CCl4 damages argyrophilic nucleolar organiser regions (AgNORs), which disperse with irregularly shaped particles and cause genotoxicity by breaking deoxyribonucleic acid (DNA) strands4. It can cause hepatic injury. Hence, it is widely used to construct as toxic liver model in rats5. Defence against oxidative harm is evolved and essentially reliant on antioxidant enzymes, substrate supply, and injury repair. These defences strengthen in response to oxidants and other electrophiles, boosting the capacity to repair oxidative damage, detoxify oxidants, and electrophiles. The main strategies underpinning antioxidant therapy are agents that boost these defences. Extensive research on antioxidant enzyme induction has focussed on regulatory mechanisms, clinical consequences, and prospective inducers with therapeutic potential6. Olea europaea L., commonly known as olive, belongs to the Oleaceae family, and is grown all over the world, with the Mediterranean region being the primary location of agricultural output accounting for over 98% of global production7. The olive tree is widely grown outside of the Mediterranean region throughout Asia, India and the Department of Physiology, University of Karachi, Karachi, Pakistan. DOI: https://doi.org/10.47391/JPMA-DUHS-S13 Abstract Objective: To examine the therapeutic effects of Olea europaea L. leaves extract on carbon tetrachloride-induced liver injury in rats. Method: The experimental study was conducted at the Department of Physiology, University of Karachi, Karachi, in July 2021, and comprised Albino Wistar male rats weighing 180-220gm. The animals were divided into control group I, carbon tetrachloride group II, Olea europaea L. + carbon tetrachloride group III and Olea europaea L. group IV. In Vitro model of hepatic toxicity was developed by carbon tetrachloride. A daily dose of 50mg/kg of aqueous extract of olive leaves was administered orally and 0.8ml/kg of carbon tetrachloride was administered twice a week subcutaneously for 28 days. On the 29th day, the animals were sacrificed, and tested for hepatic enzymes, lipid peroxidation markers and histopathology. Data was analysed using SPSS 20. Results: Of the 24 rats, 6(25%) were in each of the 4 groups. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and total bilirubin levels were significantly reduced (p<0.05) in group II whereas, 4- hydroxynonenal, isoprostane and malondialdehyde levels were significantly increased (p<0.05). However, total antioxidant level increased significantly (p<0.05) in group III compared to group II. Histopathology showed severe liver damage in group II and mild damage in group III. Conclusion: Olea europaea L. leaves extract was found to have profound hepatoprotective effects. Key Words: Antioxidants, Olea, carbon tetrachloride, alanine transaminase, alkaline phosphatase, liver injury, chronic, lipid peroxidation, bilirubin, aspartate aminotransferases, malondialdehyde, isoprostanes Downloads Full Text Article Published 2024-02-11 How to Cite Amber Ayaz Memon, & Lubna Naz. (2024). The hepatoprotective potentials of Olea europaea L. leaves against carbon tetrachloride-induced hepatic injury in rats. Journal of the Pakistan Medical Association, 74(2), S63-S67. https://doi.org/10.47391/JPMA-DUHS-S13 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 74 No. 2 (2024): 1st International Conference On Biomedical Sciences Section RESEARCH ARTICLE License Copyright (c) 2024 Journal of the Pakistan Medical Association This work is licensed under a Creative Commons Attribution 4.0 International License.