Impact of Age, Gender, post infection and post vaccination status on antibody response in COVID 19 patients Authors Sidra Sadiq Department of Chemical Pathology and Endocrinology, Rehman Medical Institute, Peshawar, Pakistan Maria Khan Department of Microbiology, Rehman Medical Institute, Peshawar, Pakistan Tayyaba Sadiq Rehman College of Allied Health Sciences, Peshawar, Pakistan Sheryar Orakzai Department of Chemical Pathology and Endocrinology, Rehman Medical Institute, Peshawar, Pakistan Mirza Muhammad Dawood Department of Chemical Pathology and Endocrinology, Rehman Medical Institute, Peshawar, Pakistan Najeeb Ul Haq Rehman College of Allied Health Sciences, Peshawar, Pakistan DOI: https://doi.org/10.47391/JPMA.5309 Abstract Objective: To evaluate severe acute respiratory syndrome coronavirus-2 spike protein antibodies against coronavirus disease-2019 in post-infection and post-vaccinated individuals. Method: The cross-sectional study was conducted June, 1 to July 31, 2021, at the Rehman Medical Institute, Peshawar, Pakistan, and comprised subjects of either gender in whom immunogenicity was checked 35 days post-vaccination and 90 days post-infection. Correlation with age and gender was checked. Specimens were collected and investigated for severe acute respiratory syndrome coronavirus-2 spike protein antibodies by consuming electro-chemiluminescence immunoassay. Data was analysed using SPSS 23. Results: Of the total 256 patients enrolled, 70(27.34%) were included; 49(69%) males and 21(29.6%) females. The overall mean age was 44±7.75 years. Among 30(42.8%) patients with positive polymerase chain reaction test, the mean time between positive the test and antibody screening was 90±30 days. Among the 40(57.2%) vaccinated individuals, the time between vaccination and antibody screening was 35±9.74 days. Overall, 68(97%) patients revealed robust positive findings to severe acute respiratory syndrome coronavirus-2 spike proteins antibodies >50IU/mL. Male subjects had significantly higher immunogenic response compared to females (p=0.001), and immunogenicity decreased with advancing age (p<0.001). Also, post-vaccinated patients’ antibody response was significant compared to post-infection patients’ response (p=0.001). Conclusion: Majority of the patients had significantly higher antibody titers against severe acute respiratory syndrome coronavirus-2 post-infection and post-vaccination. Males and younger individuals developed a significant humoral immunity compared to females and the elderly. Key Words: Antibodies, COVID-19, SARS-CoV-2, Vaccination. Author Biographies Sidra Sadiq, Department of Chemical Pathology and Endocrinology, Rehman Medical Institute, Peshawar, Pakistan TMO Chemical Pathology Maria Khan, Department of Microbiology, Rehman Medical Institute, Peshawar, Pakistan Consultant Microbiologist Tayyaba Sadiq, Rehman College of Allied Health Sciences, Peshawar, Pakistan BS MLT 5th semester Sheryar Orakzai, Department of Chemical Pathology and Endocrinology, Rehman Medical Institute, Peshawar, Pakistan TMO Chemical pathology Mirza Muhammad Dawood, Department of Chemical Pathology and Endocrinology, Rehman Medical Institute, Peshawar, Pakistan Consultant Chemical pathology Najeeb Ul Haq, Rehman College of Allied Health Sciences, Peshawar, Pakistan BS MLT 5th semester Downloads Full Text Article Published 2022-08-15 How to Cite Sadiq, S., Khan, M., Sadiq, T. ., Orakzai, S. ., Muhammad Dawood, M., & Ul Haq, N. (2022). Impact of Age, Gender, post infection and post vaccination status on antibody response in COVID 19 patients. Journal of the Pakistan Medical Association, 72(9), 1805–1809. https://doi.org/10.47391/JPMA.5309 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 72 No. 9 (2022): SEPTEMBER Section RESEARCH ARTICLE