Frequencies and results of anti-nuclear, anti-dsDNA and anti-ENA in a tertiary-care hospital in Karachi, Pakistan Authors Fatima Husain Kanani The Indus Hospital, Karachi, Pakistan Tahir Hussain The Indus Hospital, Karachi, Pakistan Tayyaba Talat The Indus Hospital, Karachi, Pakistan Nida Ghouri The Indus Hospital, Karachi, Pakistan DOI: https://doi.org/10.47391/JPMA.1301 Abstract Objectives: To analyse frequencies and results of anti-nuclear antibodies, anti-double stranded deoxyribonucleic acid and anti-extractable nuclear antigens tests ordered in a tertiary-care hospital. Method: The retrospective study was conducted at a tertiary care hospital in Karachi, and comprised all tests ordered for anti-nuclear antibodies, anti-double stranded deoxyribonucleic acid and anti-extractable nuclear antigens from March 2017 to January 2018. Data was retrieved from the institutional electronic database. The frequencies and results of the tests were determined. Anti-nuclear antibodies test was determined by indirect immunofluorescence, while the other two tests were determined by enzyme-linked immunosorbent assay. Patterns emerging from anti-nuclear antibodies tests were also analysed. Results: Of the 1053 tests ordered, 1000(95%) were for anti-nuclear antibodies.. It was positive in 260(26%) patients, and was repeated in 8(3%) of the positive and 9(1.2%) of the negative patients. Anti-double stranded deoxyribonucleic acid test was ordered in 300(40.5%) and anti-extractable nuclear antigens test in 125(17%) patients who had tested negative for anti-nuclear antibodies. Among those who tested positive for anti-nuclear antibodies, the commonly observed patterns were homogenous 109(41.9%) and speckled 103(39.6%). Rod and ring pattern was seen in 10(3.8%) patients, and none of them was on anti-viral treatment. Conclusion: There was injudicious and unjustified ordering of auto-antibodies testing, indicating the need for greater physician education and cost-effective protocols. Key Words: Auto-antibodies, Homogenous, Speckled, Rod and ring pattern, Hepatitis C, Continuous... Downloads Full Text Article Published 2020-11-17 How to Cite Fatima Husain Kanani, Tahir Hussain, Tayyaba Talat, & Nida Ghouri. (2020). Frequencies and results of anti-nuclear, anti-dsDNA and anti-ENA in a tertiary-care hospital in Karachi, Pakistan. Journal of the Pakistan Medical Association, 71(1), 708–712. https://doi.org/10.47391/JPMA.1301 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 71 No. 1 (2021): JANUARY "B" Section Audit License Copyright (c) 2020 Journal of the Pakistan Medical Association This work is licensed under a Creative Commons Attribution 4.0 International License.