Gene-gene and gene-environment interaction: an important predictor of oral cancer among smokeless tobacco users in Karachi

Authors

  • Muhammad Mohiuddin Alamgir Department of Pathology, Dow University of Health Sciences, Karachi, Pakistan
  • Qamar jamal Department of Pathology, Ziauddin University, Karachi, Pakistan
  • Talat Mirza Department of Pathology, Ziauddin University, Karachi, Pakistan

DOI:

https://doi.org/10.47391/JPMA.1806

Abstract

Objective: To determine the risk for oral cancer caused by simultaneous occurrence of more than one of the tested cytochrome P450 1A1MspI, glutathione S-transferaseM1 null gnd Glutathione S-transferasesT1 null gene polymorphisms.

Method: The cross-sectional case-control study was conducted from December 2011 to October 2016 at the Ziauddin University, Karachi, in collaboration with Dow University of Health Sciences, Karachi, and comprised oral squamous cell carcinoma cases in group A and healthy tobacco habit-matched controls in group B. All investigations were done using standardised laboratory protocols. The outcomes were determined in terms of association of various combinations of cytochrome P450 1A1MspI, glutathione S-transferasesM1 null and glutathione S-transferases T1 null polymorphisms with oral cancer. Data was analysed using SPSS 20.

Results: Of the 238 subjects, 140(58.8%) were in group A and 98(41.2%) were in group B. Mean ages in group A and B were 47.1±12.22 and 41.6±14.58 years, respectively. Male/Female ratio in group A was 1.88:1 while 83.4% were using tobacco. When cytochrome P450 1A1MspI homozygous (m2/m2) and glutathione S-transferasesM1 null variants occured simultaneously in an individual, an odds ratio of 12.8 (95% confidence interval: 1.20-135.5; p=0.03) among overall tobacco chewers was observed. For glutathione S-transferasesM1 not null and glutathione S-transferasesT1 null variant combination among overall tobacco users, the conferred odds ratio was 4.58 (95% confidence interval: 0.99-21.2; p=0.05). The other studied gene combinations did not reveal significant associations (p>0.05).

---Continue

References

Omiecinski CJ, Heuve VJP, Perdew GH, Peters JM. Xenobiotic metabolism, disposition, and regulation by receptors:From biochemical phenomenon to predictors of major toxicities. Toxicol Sci.2011; 120:S49-75.

Gundert-RemyU, BernauerU, BlomekeB, DoringB, FabianE, GoebelC, etal. Extrahepatic metabolism at the body’s internal-external interfaces. Drug Metab Rev.2014; 46:291-324.

Taspinar M, Aydos SE, ComezO, Elban AH, Karabulut HG, Sunguroglu A. CYP1A1,GST gene polymorphisms and risk of chronic myeloid leukaemia.Swiss Med Wkly.2008; 138:12-7.

KatakwarP, MetqudR, NaikS, MittalR. Oxidative stress marker in oral cancer:A review. J Can Res Ther.2016; 12:438-46.

Peddireddy V, Badabagni SP, Gundimeda SD, Mamidipudi V, Penagaluru PR, Mundluru HP. Association of CYP1A1,GSTM1 and GSTT1 gene polymorphisms with risk of non-small cell lung cancer in Andhra Pradesh region of South India. [Online] [Cited 2021 Jan 13].Available from: URL:https://link.springer.com/article/10.1186/s40001-016-0209-x

Feki-Tounsi M , Khlifi R, Louati I, Fourati M, Mhiri MN, Chaffai AH, etal.Polymorphisms in XRCC1,ERCC2,and ERCC3DNA repair genes,CYP1A1 xenobiotic metabolism gene, and tobacco are associated with bladder cancer susceptibility in Tunisian population. Environ Sci Pollut Res.2017; 24:22476-84.

Choudhury JH, Singh SA, Kundu S, Choudhury B, Talukdar FR, Srivasta S, etal.Tobacco carcinogen-metabolizing genes CYP1A1,GSTM1, and GSTT1 polymorphisms and their interaction with tobacco exposure influence the risk of head and neck cancer in North east Indian population. Tumour Biol.2015; 36:5773-83.

CroccoP, Montesanto A, Dato S, Geracitano S, Iannone F, Passarino G, etal. Inter-individual variability in Xenobiotic-Metabolizing Enzymes:Implications for Human Aging and Longevity.Genes.2019; 10:403.

Devasena A, Chaubal PC, Kannan S, Bhisey RA, Mahimkar MB. Susceptibility to oral cancer by genetic polymorphisms at CYP1A1,GSTM1 and GSTT1 loci among Indians:Tobacco exposure as a risk modulator.Carcinogenesis.2007; 28:1455-62.

Zhou T, Li HY, Xie WJ, Zhong Z, Zhong H, Lin ZJ. Association of Glutathione Stransferase gene polymorphism with bladder Cancer susceptibility. BMC Cancer [Online] [Cited 2020 Dec 23]. Available from: URL:

https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-5014-1.

Zakiullah, Ahmadullah, Khisroon M, Saeed M, Khan A, Khunda F, etal. Genetic susceptibility to oral cancer due to combined effects of GSTT1,GSTM1 and CYP1A1 gene variants in tobacco addicted patients of Pashtun ethnicity of Khyber Pakhtunkhwa province of Pakistan. Asian Pac J Can Prev.2015; 16:1145-50.

Abbas M, Srivastava K, Imran M, Banerjee M. Association of CYP1A1 gene variantsrs4646903 (T>C) and rs1048943 (A>G) with cervical cancer in North Indian population.Eur J Obstet Gynecol Reprod Biol.2014; 176:68-74.

Zakiullah, Saeed M, Ali S, Khisroon M, Muhammad B, Khuda F,etal. Genetic susceptibility to esophageal cancer due to CYP1A1 gene variants 4646903 in tobacco addicted patients of Pashtun ethnicity: a case-control study in Khyber Pakhtunkhwa province of Pakistan. Asian Pac J Can Prev.2014;15:6715-20.

Singh SA, Ghosh SK. Metabolic PhaseI (CYPs) and PhaseII (GSTs) Gene Polymorphisms and Their Interaction with Environmental Factors in Nasopharyngeal Cancer from the Ethnic Population of Northeast India.Pathol Oncol Res.2019; 25:33-44.

Varela-LemaL,Taioli E, Ruano-RavinaA, Barros-Dios J, Anantharaman D, Benhamou S, etal. Meta-analysis and pooled analysis of GSTM1 and CYP1A1 polymorphisms and oral and pharyngeal cancers:a HuGE-GSEC review. Genet Med.2008; 10:369-84.

Daniel WW. Biostatistics:a foundation for analysis in Health Sciences,5thed. John Wiley & Sons,1987.

Ada AO, Yilmazer M, Suzen S, Demiroglu C, DemirbagAE, EfeS, etal. Cytochrome P450 (SYP) and glutathione S-transferases (SGT) polymorphisms (CYP1A1,CYP1B1,GSTM1,GSTP1,and GSTT1) and urinary levels of 1-hydroxypyrenein Turkish coke oven workers. Genet Mol Biol.2007; 30:511-19.

Li W, Song LQ, Tan J. Combined effects of CYP1A1MspI and GSTM1 genetic polymorphisms on risk of lung cancer:an updated meta-analysis.Tumor Biol. 2014; 35:9281-90.

Zhang WP, He XF, Ye XH. Association between the combined effects of GSTM1 present/null and CYP1A1MspI polymorphisms with lung cancer risk:an updated meta-analysis. [Online] 2020 [Cited 2021 January 13]. Available from: URL: https://portlandpress.com/bioscirep/article/40/9/BSR20202275/226457/Association-between-the-combined-effects-of-GSTM1

Girdhar Y, Singh N, Behera D, Sharma S. Combinations of the Variant Genotypes of CYP1A1, GSTM1 and GSTT1are associated with an increased lung cancer risk in North Indian population:a case-control study. Pathol Oncol Res.2016; 22:647-52.

Published

2022-03-03

How to Cite

Muhammad Mohiuddin Alamgir, jamal, Q., & Mirza, T. (2022). Gene-gene and gene-environment interaction: an important predictor of oral cancer among smokeless tobacco users in Karachi. Journal of the Pakistan Medical Association, 72(3), 477–482. https://doi.org/10.47391/JPMA.1806

Issue

Section

RESEARCH ARTICLE