October 1994, Volume 44, Issue 10

Original Article

Screening for Chronic Lead Poisining in Lead Factory Workers

Dilshad Ahmad Khan  ( Armed Forces institute of Pathology, Rawalpindi. )
Iftikhar Ahmed Malik  ( Armed Forces Institute of Pathology, Rawalpindi. )
Muhammad Saleem  ( Armed Forces Institute of Pathology, Rawalpindi. )
Rizwan Hashim  ( Armed Forces Institute of Pathology, Rawalpindi. )
Rashid Bashir  ( Armed Forces Institute of Pathology, Rawalpindi. )

Abstract

One hundred and forty-nine lead factory workers comprising of 46 fume exposed, 78 handling lead materials and 25 controls were screened for chronic lead poisoning. Blood lead level was determined by atomic absorption spectrometery and urinary ALA by ion-exchange chromatography. Fume exposed workers had significantly higher (P<0.01) blood lead (median 61.20 ug/dl, range 21.20 - 171,10 ug/dl) and urinary ALA levels (median 4i0 mg/I, range 01.0-22.9 mg/I) than workers handling lead materials and controls. Urinary ALA was found to be a more sensitive and specific test for lead poisoning than estimation of blood lead levels (JPMA 44:239,1994).

Introduction

Lead is cumulatively toxic and one of the serious metallic poisons1. Its toxicity in occupationally exposed individuals is well recognized in smelting operations in lead furnaces, battery repairing, ship breaking, glass manufactur­ing, printing, painting and radiator repairing. Various studies carried out in these vocations showed lead toxicity in the range of 39%-47%2-5. Lead inhibits the heme synthetic pathway by affecting 5-amino levulinic acid (ALA) dehydrase enzyme and increas­ing ALA levels in plasma to the extent that ALA starts to get excreted in urine. Urinary ALA is used for screening of lead poisoning in exposed worker6 and has been found to correlate with serum lead levels7. Persistent increase in urinary ALA 5-amino levulinic acid due to lead exposure is also advantageous for testing. As lead leaves the blood to be stored in the skeletal system, a random blood sample may show a misleading normal level. hi contrast urinary ALA remains increased for longer duration in lead exposed worker8. The condition of lead exposed industrial worker in Pakistan is worse due to ignorance, lack of safety measures, poor industrial hygiene and deficient resources. This study was planned to screen for chronic lead poisoning in different groups of adult males exposed to lead fumes and lead material in workers of a factory using lead metal.

Patients and Methods

The study was carried out from July, to December, 1993 at the Armed Forces Institute of Pathology, Rawalpindi. A total of 149 subjects aged 18-59 years were included in the study. They were grouped in three categories.
1. Lead Fume Exposed: Forty six lead furnace workers of a factory were studied. They were exposed to lead fumes released from a smelting operation. Work duration was 8-12 hours per clay with the length of service ranging from 2 to 38 years. These welt further sub-grouped into less than 10 years and morn than 10 years of service.
2. Lead Handlers: Seventy eight workers of a factory handling lead pellets who were not exposed to fumes were included. Their length of service varied from 2-28 years. These were further sub grouped to less than 10 or more than 10 years of service.
3. Controls: Twenty five healthy, age and service matched men not exposed to any known souree of lead were also included in this study.
A) Blood: Ten ml blood was collected for lead estima­tionin heparinized tubes from each individual away from their place of work Blood lead levels were estimated by Hitachi AAS-180-80 atomic absorption spectrometer with Zeeman correction mode using acetylene flame as atomizer9.
B) Urine: Ten ml of mid thy urine samples were collected in amber bottles, containing 0.1 ml glacial acetic acid. The urine samples welt stored in refrigerator at 4°C. Urinary ALA estimation was done using ALA kit (Board Germany). Urinary ALA elution was carried out by ion exchange chromatography column test and eluted ALA was measured by colorimetric method7. The median and range of blood lead and urinary ALA in controls and different groups of industrial workers were calculated. Wilcoxan Rank sum test was applied for statistical analysis of that.

Results

Exposed workers were grouped into three categories according to blood levels. They were considered as normal if levels were less than 50 ug/dl; acceptable to continue work with necessary precaution if blood lead levels were between 50-70 ug/dl and unacceptable if levels were more than 70 ug/dl11. The relationship between lead exposure and urinary ALA levels was considered according to de Bruin and workers and were grouped as: normal with urinary ALA levels of 0-4.5 mg/dl, mild toxicity at a range of 4.6-10 mg/dl, and moderate to severe when levels were more than 10 b Lead fumes exposed workers were found to have significantly higher blood lead (median 61.2 ug/dI) and the urinary ALA levels (median 4.1 mg/I) as compared to controls (Table I).

Mild lead toxicity was found in 32.6% and moderate to severe in 10.8% of fume exposed workers. Urinary ALA estimation revealed that 15 (32.6%) had mild (urinary ALA level <10 mg/I) 5 (10.9%) had moderate to severe (more than 10mg/I) lead toxicity (Figure).
Workers handling lead pellets had moderately raised urinary ALA (median 3.9 mg/l) and blood lead levels (median 42 ug/dI) compared with controls (Table II).

In lead handlers 38.4% had mild toxicity and the rest no toxicity (Figure).

Lead fumes exposed workers of morn than 10 years of service had significantly elevated urinary ALA levels as compared to those having less duration of service (Table III).

Similar findings were also observed in lead handlers. How­ever, the blood lead levels did not show any significant change with the duration of exposure in both groups.

Discussion

The present study was carried out in workers exposed to lead fumes and those handling lead pellets showed that the fonner had a higher urinary ALA (median 4. 1) and blood lead levels (median 61.1) as compared to controls (Table IV).

In a study from Karachi moderately high blood lead levels were reported in traffic policemen due to exposure to lead fumes in low concentrations12. Mild lead toxicity in 32.6% and moderate to severe in 10.8% cases of those exposed to lead fumes might be due to direct absorption of lead from the lungs. Sarah et al. 13 determined blood lead levels in indoor firing range users exposed to lead fumes. Their blood lead levels ranged from 6.4 to 51.1 ug/dl and these levels were low when compared to lead furnace shops workers. Lead is absothed from the alveoli of lung with nearly 90% efficiency14 and high blood levels in lead fume exposed workers in our study was probably due to poor working conditions, lack of knowledge and absence of preventive measures for personal protection. Workers handling lead pellets with bate hands had moderately raised blood lead levels (median 42 ug/dl) and 38.4% had mild lead toxicity. These results were consistent with studies conducted on occupationally exposed lead battery workers and ship scrapping employees whose blood lead levels were over60 ug/dl2,4. Automobile radiator mechan­ics handling lead materials also had elevated levels ranging from 16-73 ug/dl with 39% mechanics showing levels greater than 40 ug/dl3. Workers exposed to lead fumes and lead handlers showed significant correlation between the duration of expo­sure and their urinary ALA levels but no significant change was noted in their blood lead levels. Similar findings were reported by others3,15. Unless blood lead values are determined within a relatively short time following exposure to lead, they may be of little help in any screening programme designed for detecting lead exposure in asymptomatic individuals. Meas­urement of urinary ALA values completely eliminates this disadvantage since urinary ALA levels remain increased for prolonged periods following removal of the individual from the source of lead exposure7. Urinary ALA test has shown closest correlation with the clinical picture of lead poisoning when compared to all other currently available major laboratory tests16-19. It is concluded that chronic lead poisoning was more common in woilcers who were exposed to lead fumes because they had significantly higher blood lead and urinary ALA levels as compared to lead handlers and unexposed healthy adults. The urinary ALA estimation is a more sensitive and specific test than blood lead levels for testing for lead toxicity.

References

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