March 1995, Volume 45, Issue 3

Original Article

Blood Lead Levels and Anemia in Lead Exposed Workers

Rashid Bashir  ( Department of Medicine, POF Hospital, Wah Cantt. )
Dilshad Ahmad Khan  ( Armed Forces institute of Pathology, Rawalpindi. )
Muhammad Saleem  ( Armed Forces Institute of Pathology, Rawalpindi. )
Khursheed Uz Zaman  ( Department of Medicine, POF Hospital, Wah Cantt. )
Iftikhar Ahmad Malik  ( Armed Forces Institute of Pathology, Rawalpindi. )

Abstract

The effects of lead on haematological parameters were studied in 51 occupationally exposed individuals comprising of 27 lead furnace workers, 24 lead pellet handlers and 20 healthy age and service matched controls. Blood lead levels were estimated by atomic absorption spectrometer AAS-180-80 and haemato­logical parameters by Technicon H.1TM system. The lead furnace workers had highest blood lead levels (median 71.20 ug/dl, range 21.2- 171.10 ug/dI) and low Hb (median 106 g/l, range 73-144 g/l) as compared to healthy subjects (median lead levels 29.80 ug/dI, range 10.20-54.10 ug/dI and Hb median 135 g/l, range 101-153 g/l). The workers handling pellets had moderately increased blood lead levels (median 45.50 ug/dl, range 8.50-130.6 ug/I) and low Hb (median 114 g/l, range 74-158 g/l). The furnace exposed workers had higher blood lead levels and low Hb compared to the lead pellet handlers. TRBC, Hct, MCV, MCH and MCHC did not reveal any significant difference in all groups. It is concluded that chronic lead exposure causes nonnocytic normochromic anaemia and shows a dose response relationship between lead levels and severity of anemia (JPMA 45: 64, 1995).

Introduction

Lead is a cumulative toxic metal and is known for its toxicity since time immemorial. It is rapidly and efficiently absorbed from respiratory tract (nearly 90%) as well as from gastrointestinal tract (l0-40%)1. It acts at various steps interfering with the production of heme, causing anaemia. There are conflicting views on the type of anaemia encoun­tered in chronic lead intoxication. Some believe that it is of hypochromic microcytic while others maintain that it is normocytic normochromic1-3. To assess that increasing expo­sure to lead causes increasing anaemia, Schwartz et al4 conducted an epidemiological study and concluded that there is dose related depression of hematocrit in their subjects. Similarly Veemla and Noah5 in their study of 58 patients of lead poisoning, observed that those with lower haemoglobin values had relatively higher blood lead levels. There have been veiy few studies on lead intoxication in Pakistan, though millions are exposed to lead. This study was done on lead exposed workers, to evaluate the degree of their exposure to lead, by determining blood lead levels and to see the degree and type of anaemia caused by chronic lead exposure.

Material and Methods

The study was conducted from January to May, 1993 in a factory, where lead is extensively used in the manufacturing of pellets and other material. A total of 51 workers were divided into two groups according to the risk involved. A control group was also included.
High Risk- Group I
Twenty-seven workers, ages ranging 22-55 years ex­posed to lead fumes while working on lead melting furnaces 8-12 hours/day with no safety measures were included in this group. The duration of exposure was from into 28 years.
Moderate Risk-Group II
Twenty workers ages ranging 24-56 years, who handled lead pellets and bullets with bare hands, without any safety precautions, were included in this group. The duration of exposure was from 5 to 35 years.
Controls
Twenty healthy, asymptomatic men from a similar factoty who were not exposed to any known source of lead were in this group. Their ages ranged from 22 to 59 years and service duration from 2 to 30 years.
Blood Sampling and Tests
Subjects were sampled away from their place of work, to avoid contamination. Antecubital vein was used for standardization. Heparin was used as anticoagulant. Blood lead levels were determined on Hitachi Atomic Absorption Spectrometer-AAS-180-80 with Zeeman correction mode, using acetylene flame as atomizer. Complete blood counts were done at Armed Forces Institute of Pathology, Rawalpindi on Technicon HI System to obtain Hb, Hct, TRBC, MCV, MCH and MCHC.
Statistical Analysis
The sample size did not follow the Gaussian distribu­tion, therefore Wilcoxon Rank Sum test was used for comparison.

Results

Both the high risk and moderate risk groups had significantly elevated blood lead levels as compared to controls (Tables I and II). Fume exposed workers (Table I) had higher blood lead levels ranging from 21.2 to 171.1 ug/dl (median 71.2), compared to 10.2 to 54.1 ug/di (median 29.8) in controls. Significant anaemia was also seen in both exposed groups with median Hb levels of 106 g/l in high risk and 114 g/I in moderate risk, as compared to 135 g/l in control group. Anaemia was more marked in high risk group as compared to moderate risk but the difference is not statistically significant (Table HI). However in all three groups when compared (Tables I, II and ifi), there was nO difference in the values of MCV, MCH and MCHC, showing that the anaemia was of normochromic, normocytic type. When blood lead levels were correlated to haemoglobin (Figure), there was significant (P<0.01) regressing correlation between the two. That is, as the blood lead level increased the haemoglobin concentration fell.

Discussion

We have found a few peculiar results in our study. The control group values of blood lead concentration, haemoglo­bin and other parameters were within nonnal limits. The median blood lead’ level was 29.8 ug/dl, while 40 ug/dI is the WHO recommended upper acceptable limit in adult male workers6. However, in USA, the Centers for Disease Control in 1985 stated a level of25 ug/dl to be considered as elevated7.
Anaemia (Tables I and II)


is the main finding in this study, withHb, TRBC and Hct below the lower nonnal limits. This is consistent with all reported studies3,8,9. Anaemia due to lead poisoning is of hypochromic, microcytic type. How- ever, a few authors believe it to be normochronuc, nonno­cytic2,3. We have found a normocytic, normochromic anaemia (Table I and II), with MCV, MCH and MCHC no different from the normal values.The causes of anaemia in chronic lead exposure are many. Lead suppresses many enzymes in the process of heme synthesis1, decreases life span of red cells and delays regeneration of red cells10.Lead affects red cell membrane enzymes and pro­teins3,11-13 and increases hemolysis14. The microcytic anemia usually found in lead exposed individuals may be due to co-existing iron deficiency.

The results shown in Figure are similar to other studies4,5. A strong dose response relationship is shown between anaemia and blood lead levels. Decreasing haemo­globin and haematocrit are seen with increasing blood lead levels. However, duration of exposure to lead is an important factor in the causation of anaemia15. Acute exposure does not cause anemia, it has to be chronic. Occupational exposure to lead in Pakistan is still unchecked, causing high blood lead levels and significant anaemia of nonnocytic normochromic type. Furthermore, there is a dose response relationship between lead levels and severity of anaemia.

References

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