March 1996, Volume 46, Issue 3

Original Article

Evaluation of Paromomycin Sulphate Topical Ointment as Effective Therapeutic Agent in Cutaneous Leishmaniasis

N. J. Sahibzada  ( Dermatology, Bolan Medical College, Quetta. )
A. K. Tareen  ( Dermatology, Bolan Medical College, Quetta. )
Tariq Khursheed  ( Departments of Pathology, Combined Military Hospital, Quetta. )
Nasar Darr  ( Departments of Pathology, Combined Military Hospital, Quetta. )

Abstract

This is a prospective open study of randomly selected 35 patients with a single sore of cutaneous leishmaniasis who were treated with paromomycin sulphate topical ointment for 4 weeks. According to the observationsmade on days 0,15,45 and 105 after the careful application twice a day in 2 lengths from one side of the lesion to the other at right angles to each other and then smeared to cover the whole surface of the sore, the overall efficacy of the ointment as therapeutic agent was 91%. There was a mild and temporary adverse reaction in the form of painless, non-itching nodulation around the ulcers in 10(28%) patients after 15 days application which disappeared within 5-7 days of furtherapplication. It thus proved a promising, simple and inexpensive remedial agent without any undesirable side effects as compared to other complicated and unpredictable therapeutic regimens (JPMA 46: 53,1996).

Introduction

Cutaneous leishmaniasis (CL) famously known in the world by the regional or local names such as Oriental sore, Delhiboil, Allepo boil, Baghdad Boil, Biskra boil and Quetta/Kandahar Kaldana prevails over several areas with different geographical conditions in the province ofBalochis­tan. There was a time when the natives of Quetta (Provincial capital), Kasi, Pathans and Berahvis and people living in Kandahar (Afghanistan) were recognised in other areas of the region by the presence of disfiguring scars on their faces and other exposed parts of the body. The lesion has since been named Kaldana in Pashto in Balochistan and Afghanistan meaning a sore taking years to heal1,2. Balochistanbeing at the crossroad of Afghanistan in the North, Iran in the West. India in the East and Arabian Sea in South had been in the past and is still exposed to Leishmanial diseases through trvellers entering it’s frontiers from endemic areas. The endemicity of CL has achieved greater dimensions in the recent years in Balochistan and the disease may well be placed on the top of the list of patients with skin disorders attending the dispensa­ries and hospitals both in government and private sectors in rural and urban areas (unpublished).
Due to lack of effective vector control and other prophylactic measures in a hyperendemic region like Balo­chistan where poverty, illiteracy and lack of civic sense prevail, these problems became more alarming through the continuous huge influx of Afghan refugees. who entered the province through its northern frontiers for the last 15 years. Besides other sufferings, both CL and visceral leishmaniasis (VL) were introduced on a large scale and the control of the disease has become a major health problem. The unavailabil­ity, toxicity, parenteral painful and uncontrolled administra­tion of conventional pentavalent antimonials and other old fashioned therapies in the scarcely populated rural areas here prompted us to resort to the use of a topical ointment contaning 15% paromomycin sulphate as a remedial agent which was used in the cases of recurrent cutaneous leishmaniasis (LR) by El-On et al 19853-5. The same ointment is used in the University College Hospital London. The ointment was used in 1990 for CL sores for 3 weeks with apparently complete clinical cure. In another study the same ointment was used in 100 cases of CL with 80% cure rate6. The objective of the study, is to assess improved curative measures by exploring. the efficiency of the topical ointment and observe any side effects.

Patients and Methods

Thirty-five adult male patients with a localised single sore less than or equal to 5 cm in diameter, which persisted for less than 4 months andlocated 3 cin away from the eyes, were included in the study after obtaining a written consent. These patients had not received any treatment before. There was no history of allergy and no concomitant serious disease was present. Their ages ranged from 17 to 39 years.
The diagnosis was made by skin slit preparation technique and after staining the smears with Giemsa stain, when leishmanial amastigotes were seen in the dermal macrophages or disbursed extracellularly. The patients were examined for physical fitness and laboratory investigations such as leucocyte count, haemoglobin. blood urea nitrogen and SGOT were done on day zero, 15. 45 and 105. Photo­graphs of the sores were taken on the same days (Figures Ia. and Ib).



Two tubes of topical ointment each containing 30g (11.50% Paromomycin) were labelled for everv patient and it squeezingjjfrbm the collapsible tube in2 lengths over the sore airight angle from one another.
Partial response or apparent cure was evaluated by dryness or otherwise of the sore, epithelization, decrease in size and edema and lowering of edges, smear positivity/nega­tivity and tolerability or adverse reaction on days 15,45 and 105.
All of the patients were soldiers in the Pak Army based at Quetta and they were deputed to undergo an exercise for 2 months in a dry river bed (Nari bank) near Sibi which is an L-major hyperendemic region in Balochistan. After the exercise was over they were sent home on leave. Almost all of them belonged to northern districts of the country and they were non-immune to the disease. About 1300 personnel were bitten by the vector and these were notified in the Combined Military Hospital. Quetta during a period of 7 months (March to October, 1993).
Their ages ranged from 17 to 39 years and average duration of the sore development was one and half month. All of them had a localized sore on the exposed parts of the body. less than 5 cm in diameter.
The white cell count, hacmoglobin, blood urea nitrogen and SGOT, done on day 0,15,45 and 105 were observed to be within normal limits.
On day 15, partial response of apparent cure was observed as dryness of the sore, epithelization, decrease in size and edema and lowering of edges in 3(9%) patients (Figure 2a and ib).


There was a mild and temporary adverse reaction in the form of painless and non-itching nodulation around ulcers in 10 (28%) which disappeared within 5 to 7 days of further application (Figure 3a. b and c).


The same parameters were evaluated on day 45 with clinical assessment of efficacy revealed by clinical improvement in 26 (74%) patients On day 105, final observations were made by evaluating the clinical as well as parasitological cure in 32 patients (91%).
(Figure 3a and 3b). During the follow-up period of 6 months, no recurrence was observed.

Discussion

Cutaneous leishmaniasis is widely spread throughout Balochistan due to it’s strategic geographical situation within Irano- Turanian (40%) and Saharo-Sindian regions of the world where there is maximum distribution of L-major disease. The disease occurs sporadically throughout the year, the explosive human infection occurs in epidemics. First epidemic occurred in 1935, after a dreadful earthquake in Quetta, when thousands of children were affected. The subsequentepidemics occurred inMultanin 1971-72 inwhich 2500 patients were affected followed by epidemics in the year 1976-77 inUthal, 1979-80 inPirkuh(Sibi), 1990 in Quetta and
1993 -94 in Sibi when diggings were done on large scale for the development of industzy and towns whereby non-immune workers were exposed to the bite of the vector in those areas.
Zoonotic CL due to L. major is the main type of leishmanial disease in Balochistan and rest of the country, except in Mirpur in Mangla Dam area and a spot in the extreme south of Balochistan where from ACL due to L. Tropica has been reported.
The old remedies in this country are intralesional mepacrine. curettage and surgical removal of the non-healing, annoying and incapacitating or mutilating sores, as well as antimonial compounds parenterally and intralesionally7. The results of this study are in partial agreement with that carried out in Iran8 (Personal communication). Paromomycin is an aminoglycoside antibiotic used under the trade name of humatin in amoebiasis and intestinal tapeworm infection. It has an antibacterial activity against normal and pathogenic microorganisms inthe intestinal tract and is ineffective against extra-intestinal disease8. As aparasiticidal it inhibits synthesis irreversibly at 3 steps. It interferes with peptide formation initiation complex, it induces misreading of the code on mRNA template resulting inthe correct amino acid incorpora­tion into the peptide and it breaks up polysomes in nonfunc­tional monosomes.
By these mechanisms paromomycin sulphate interferes with integrity of the leishmanial parasites. The adverse locals reaction in the form of appearance of nodules orbiebs around theulcerandallergic indurationof its margins infew cases was transitory, painless and disappeared promptly.
It was thus concluded that Paromomycin Sulphate topical ointment is an effective, non-toxic, cost effective and simple to use therapeutic agent for cutaneous leishmaniasis.

References

1. Naimatullah, J.S., Burney, M.I, Lari, FA. et al. Cutaneous leishmaniasis in Pakistan. Clinical pattern, immunology and epidemiology. MoP-7-4, Excerpta Medica. International Congress series 810,1988.
2. Naimatullah, J.S., Burney, M.I., Lari. F.A. et al. Cutaneous leishmaniasis in Pakistan. Clinical pattern, immunology and epidemiology. Sixth International Congress on Rapid Methods and automation in microbiol and Immunol Helsinki, 1990, Abstract No. 358.pp. 209.
3. Bryceson, A. Therapy of tropical skin dIsease, Clin Exp. Dermatol., 1981;6521-525.
4. Dogra. J., La!, B.B. and Misra, S.N. Dapsone in the treatment of cutaneous leishmaniasis. Int J. Dermatol., 1986.25:398-400.
5. EL-On 7., Weinrauch, L. and Livshin, R.L. Topical treatment of recurrent cutaneous leishmaniasis with ointment containing paromamycin and methyl­benzathonicum choloride. Br. Med. 7., 1985;291:704-705
6. Naimatullah, J.S., Tareen, A.K. Evaluation of paromomycin sulphate topical ointment as effective therapeutic agent in cutaneous leishmaniasis. TuP8-14 Presentation in XIII International Congress for Topical Medicine and Malaria. 1992.
7. Olliaro, P.L and Bryceson, A.D.M. Practical progress and new drugs for changing pattern of leishmaniasis. Parasitology today, 1993 ;9:323-328.
8. Woolfe, 0. The chemotherapy of amoebiasis. In, Progress in Drug Research, Vol 8 (Jucker, E;ed) Base!, Berkhauser Verlag, 1965, pp. 11-52.

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