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June 2001, Volume 51, Issue 6

Original Article

P53 Mutations in Carcinoma Breast - a Clinicopathological Study

T. Khaliq  ( Department of Surgery, Pakistan Institute of Medical Sciences. Islarnabad. )
S. Afghan  ( Department of Surgery, Pakistan Institute of Medical Sciences. Islarnabad. )
A. Naqi  ( Department of Surgery, Pakistan Institute of Medical Sciences. Islarnabad. )
M. H. R. Haider  ( Department of Surgery, Pakistan Institute of Medical Sciences. Islarnabad. )
A. lslam  ( College of Physicians and Surgeons Pakistan. Karachi. )

Abstract

Objectives: To find out the incidence of p53 mutations and their possible correlation with clinicopathological presentation in females with breast carcinoma.
Settings: Department of Surgery, Pakistan Institute of Medical Sciences, lslamabad.
Patients and Methods: Seventy four patients with operable carcinoma breast that underwent mastectomy were included in this prospective study. Tumour tissue specimens and peripheral blood samples were examined for p53 mutations. Age, tumour size, nodal status and histopathology was assessed in patients with and without p53 mutations.
Results: Ten (13.5%) patients showed p53 mutations in their tumour specimens while 64 (86.48%) had normally functioning p53 gene. Patients were divided into two groups, A (normally functioning p53), and B (mutated p53). Intraductal carcinoma was the most frequent histological variant(A=57, B=10), while lymph nodes were involved in 67.19% (A=47) and 60%(B=6) cases respectively.
The age of patients and clinical parameters (tumour size, nodal status and histopathological diagnosis) were compared between the two groups and no statistically significant correlation between p53 mutations and clinicopathological parameters was found.
Conclusions: It was concluded that p53 mutation is present in carcinoma breast in Pakistani population but there was no significant correlation between p53 mutation and tumour aggressiveness (size, nodal status and histopathology) (JPMA 51:21 0;2001).

Introduction

Carcinoma breast is the commonest tumour and the leading cause of death worldwide1 and is also the commonest malignant tumour in Pakistani females2,3.
Of the various risk factors known or believed to cause cancer (smoking, diet, alcohol, environment and life style), genetics in the recent years have reached the attention of most clinicians and researchers. Molecular biologists now believe that tumour suppressor genes are central to the development of most, if not all tumours in human beings4-6.
p53 appears to be involved in up to 50% of human tumours, with the highest incidence in lung and gastrointestinal tumours6-9 and a significant number of breast cancers10-14 Breast tumours with p53 mutations have been shown to be more aggressive and present at a more advanced stage15-17. Although some studies have shown positive correlation, others deny any significant association between p53 mutations and clinicopathological features of the diseas&8.
This study was undertaken to see the difference in clinical presentation; (regarding age, tumour size, nodal status, metastasis and histopathology), of patients exhibiting p53 mutations, from those without p53 mutations.

Patients and Methods

This study was carried out at the Pakistan Institute of Medical Sciences, Islamabad in collaboration with A. Q. Khan Research Laboratories (Bio-engineering and Genetics Division). Seventy four female patients with operable breast cancer were included in the study. The medical and pathological data regarding stage of the disease, number of lymph nodes involved, histopathology and presence or absence of p53 mutations, was collected.
Patients were staged according to the American Joint Commission Staging System (TNM). The histopathological diagnosis was reached by trucut, excision or incision biopsy of the tumour and later confirmed by repeat histopathology of the excised tumour and involved lymph nodes, if any. Modified radical mastectomy was performed in all the cases. A tumour specimen was taken immediately after excision and frozen at -70 degree Celsius and later sent to the bioengineering and genetic Laboratory for screening of p53 mutations, along with peripheral blood sample of the same patient.
The clinicopathological parameters of the patients with p53 mutations were compared with that of the patients not exhibiting such change, with the help of student t test, and ā€˜pā€™ value of < 0.05 was considered significant.

Results

Topographic genotyping of tumour tissue specimens showed p53 mutations (base substitution) in 10 (13.5%) cases. Peripheral blood sample. mononuclear cell DNA analysis did not reveal p53 mutations in any of the cases.
After gene mapping. patients were classified into two groups, (A) those with normal p53, and (B) exhibiting p53 mutations.
Mean ages of these patients were A=46.3. B=49.lyears with a standard deviation of A=12.37, B=13.54, 95% Cl (A43.l, B=39.4), difference of mean 2.7 years (95% CI -5.8), and p >0.52 (not significant).
Regarding the tumour size, mean was A=4.5 cm, B=5.l cm, Standard deviation A2.2 cm, B= 1.7 cm, 95% CI A=3.9- 5.1 cm, B3.8-6.3 cm and difference of mean with df 72= 0.56 cm with the 95% CI of -0.94-2.06 cm., p>0.45 (not significant).
Clinical presentation, lymph node involvement and tumour histopatholgy of all the cases is presented in tables land 2.



Most of the patients (A=62, B=8), presented with a painless lump in the breast, followed by (A=35, B=5) and (A8, B=1) having pain and nipple discharge respectively.
Upper, outer quadrant was involved in most of the cases (A=37, B6). There was no family history of breast cancer disease in any of the patients At 3 months follow up. 7 patients were alive with no evidence of the disease and 3 were lost to follow up. No significant correlation was found regarding the clinicopathological features of the disease in the two grouPS.

Discussion

Recently, p53 mutations have been implicated in a number of human malignancies including breast carcinoma5. p53 belongs to the class of tumour suppressors and it occupies a pivotal role in the control of deciding the fate of the cells, especially those who have been stressed. So the genetically damaged cells owe their removal to the functioning normal p53 gene and damage to the gene itself may allow the cells bearing other mutant genes to proliferate unchecked.
Alteration of the p53 gene and its encoded protein is the most frequently encountered genetic event in human malignancy. p53 is a phospoprotein containing 393 aminoacids, it resides in the nucleus of the cell and is located on the short arm of the human chromosome 17, band 13 and is approximately 20 Kilo bases in length.
The spectrum of mutations at the p53 locus indicates that different environmental mutagens are likely to be involved18. Keeping in view the involvement of p53 in carcinogenesis as described by over 10,000 papers published so far, it was reasonable to expect p53 mutations in Pakistani breast cancer patients.
Despite the vast amount of information now available, it must be stressed, that little is surely known about the exact mechanism of p53 function. Recently, homologues of p5319-22 and an mdm-2 related protein (mdm-x)23 have been described to have important implications. It seems possible that these homologues make up a family of molecules with similar effects but induced by different signals and therefore play fundamentally different roles in cellular hemostasis.
It can be concluded, that if any adverse prognostic effect is present, it probably is too small to be depicted in this study conducted on a limited number of cases and that more studies are required to confirm its role, although recent studies even with a significantly large number of patients of breast cancer have failed to confirm any prognostic significance of p53 mutation. This may be, because of the fact that, malignant transformation has already occurred indicating a fundamental breakdown of cellular control and there is no good reason to believe, whether the p53 mutations have or have not contributed to this process, especially when the patients rarely present at an early stage of the disease, so was the case in our study, with most of the patients presenting in stage II and III.
p53 mutations are not a consistent finding in breast cancers of the same histopathologic nature. The sensitivity of this approach is poor, but the specificity is very high and p53 mutation screening in suspicious specimens may prove to be a useful adjunct to morphological assessment. Interest is building in the detection of p53 mutations in blood cells as a marker of residual disease after surgery, but encouraging reports in this regard are still awaited.

Acknowledgements

Thanks are due to the people at the Bio-engineering and Genetics Division of A.Q. Khan Research Laboratories for their technical support in topographic genotyping of tumour specimens.

References

1.Greenbiati MS. Bennett WP, Hollestein M, et al. Mutations in the p53 tumour suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res., 1994: 54: 4855-78.
2.Pakistan Medical Research Council Cancer Study Group. Frequency of malignant tumours in seven centres of Pakistan, JPMA 1977:27:335-9.
3.Ynusaf F, Jafarey MA. On the incidence of malignant tumours of breast among females in Pakistan, J. Pak. Med. Res., 1985:24:124.
4.Bishop JM, Weinberg RA (eds) , Scientific American Molecular Oncology. New York, Scientific American incorporated. 1996.
5.Hollstein M. Rice K, Greenhlatt MS, et al Sorlie Total. Database of p53 gene somatic mutations in human tumors and cell lines. Nuclic acids Res 1994 22:3551/3 -5.
6.Remvikos Y, Tominaga 0, tlammel P. Increased p53 protein content of colorectal tumors correlates with poor survival Br. J. Cancer., 1992: 66: 758-64.
7.Boasan S. Viale G, Bossi P. ci al, Cytoplasmic accumulation of p53 protein An independent prognostic indicator in colorectal adenocarcinoma Nat. Cancer inst., 1994:96:681-7.
8.Martin HM, Filipe Ml. Morris RW, et al. p53 expression and prognosis in Gastric carcinoma. lnt. J. Cancer., 1992: 50: 859-62.
9.Mitsudomi 1, Oyama 1, Kusano T. ct al. Mutations of the p53 gene as a predictor of poor prognosis in patients with non-small cell lung cancer . J Nat. Cancer. Inst., 1993: 85. 2018-23.
10.Davidoff Am, Herndon JE II. Glover NS, et al. Relation between p53 overexpression and established prognostic factors in breast cancer. Surgery., 1991:110:259-264.
11.Thor AD. Moore DH II. Edgertoti SM, et al. Accumulation of p53 tumour suppressor gene protein: an independent marker of prognosis in breast cancers. J. Nat. Cancer. Inst., 1992:84:845-855.
12.Rosanelli GP, Steindorfer P. Wirnsberger GH. et al.Mutant p53 expression and DNA analysis in human breast cancer comparison with conventional elinicopathological parameter. Anticancer Res., 1995:15:581.586.
13.Borg A, Lennerstrand J. Stenmark-Askmalm M, et al. Prognostic significance of p53 overexpression in primary breast cancer: A novel luminomeiric immunoassay applicable ott steroid receptor cytosis. Br. J. Cancer., 1995:71:1013-1017.
14.Truda H. Hirohashi S. Association among p53 mutations, nuclear accumulation of p53 protein and aggressive phenotype in breast cancer. Int. i. Cancer., 1994:5 7:498-503.
IS.Faille A, Dc Cremnoux P, Extra JM, et al. p53 mutations and overexpression in locally advanced breast cancers. Br. J. Cancer.1994:69:1145-1150.
16.Isola J, Visakorpi T, Holl i K, et al. Association of overexpression of tumour suppressor protein p53 with rapid cell proliferation and poor prognosis in node negative breast cancer patients. J. Nat. Cancer. Inst., 1992:84:1109-1114.
17.Molina R, Segui MA, Climent MA, et al. p53 oncoprotein as a prognostic indicator in patients with breast cancer. Anticancer Rcs.,1998:18:507-511.
18.Levi ne AJ P53, the cellular gatekeeper for growth and division. Cell, 1997: 88: 323-31.
19.SchmaIc H. Bamberger C. A novel protein with strong homology to the tumour suppressor p53 Oncogenc. 1997: 15 1363-7.
20.Diwell SP, Flail PA The p53 tumor suppressor gene and tumor prognosis: is their a relationship2ā€™ J, Pathol., 1995:177:221-4.
21.Saunders CM, Baum M. The breast. In: Russel RCG, William NS, Bulsirode CJK (eds). Bailley and love Short Pracitce of Surgery. London:Arnold.2000.76 -62.
22.Greenall MJ Cancer of Breast. In: Morris Pt, MaliRA(eds). Oxford text book ofsurgerv Newyork: Oxford university Press Inc. 1994 810-14.
23.Rassol Ml, Malik IA, Luqinan M. et al. A comprehensive study of breast cancer PJMR (Pakistan Journal of Medicine Research), 1998:79:2-8.

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