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May 2022, Volume 72, Issue 5

Research Article

Effect of nutritional status on wound healing after coronary artery bypass graft (CABG) surgery

Muazama Zaffar  ( Pakistan Naval Hospital, PNS Hafeez, Naval Complex, Islamabad, Pakistan. )
Asma Afreen  ( Department of Environmental Design Health and Nutritional Sciences, Allama Iqbal Open University, Islamabad, Pakistan. )
Muhammad Waseem  ( Armed Forces Institute of Cardiology /National Institute of Heart Disease (AFIC/NIHD), Rawalpindi, Pakistan. )
Zaheer Ahmed  ( Department of Environmental, Nutritional and Health Sciences, Allama Iqbal Open University, Islamabad, Pakistan. )


Objective: To assess the pre-operative nutritional status and its effect on wound healing of patients having undergone coronary artery bypass graft surgery.


Method: The hospital-based prospective study was conducted at the Armed Forces Institute of Cardiology/National Institute of Heart Disease, Rawalpindi, Pakistan, from October 2017 to March 2018, and comprised patients who underwent coronary artery bypass graft surgery. Sternal wound healing as an outcome measure was assessed with nutritional status. Investigation of dietary data focussed on various food groups and their impact on wound healing. Data was analysed using SPSS 21.


Results: Of the 330 patients, 16 (4.8%) died within hospital. The final sample, as such, comprised 314(95.2%) patients; 273 (86.9%) males and 42 (13.1%) females, with an overall mean age of 56.6±8.3 years. The mean body mass index was 26.9±4.3kg/m². There was non-significant relationship between body mass index and wound healing (p>0.05). Significant relationship was observed between albumin, blood glucose and wound healing (p<0.05).


Conclusion: Low levels of albumin and poor blood glucose control before surgery was found to be associated with delayed wound healing. Nutritional intervention prior to coronary artery bypass graft surgery may lead to reduced risk and complications during / after surgery.


Keywords: Coronary artery bypass graft surgery, Body mass index, Sternal wound infection, Nutritional status. (JPMA 72: 860; 2022)





Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality all over the world. The World Health Organisation (WHO) estimates showed that during 2013 alone >17.3 million people died from CVDs which represents 31% of deaths globally.1 Among them, 6.5 million deaths were due to stroke and 7.4 million were due to coronary artery diseases (CADs).2 Coronary artery bypass graft (CABG) procedures are the proven therapies for the treatment of ischaemic heart diseases (IHDs) and about 200,000 CABG procedures are annually performed in the United States.3 Percutaneous coronary intervention (PCI) is considered an urgent procedure for CADs, but CABG may still be needed for some patients to treat CADs.4 Secondary prevention of CABG surgery, including diabetes mellitus (DM), hypertension (HTN), weight-loss and cardiac rehabilitation, plays an important role in the prevention of cardiac mortality and post-operative outcomes.5

Relationship of body size and CABG surgery has been an ongoing debate during the last decades. Body mass index (BMI) in most surgical procedures has been defined as the indicator of malnutrition. In cardiac surgery, patients with pre-operative reduced food intake, unintentional weight-loss and BMI >40 or <20 are at higher nutritional deficiency risk.6 Gao, Sun7 observed that obesity is positively associated with deep sternal wound infection (DSWI), need for dialysis, prolonged ventilation time, mortality and readmission. However, some studies also indicated that obesity or BMI >35 is considered to be a risk factor for CADs and obese patients requiring CABG surgery showed a strong association between BMI and morbidity post-surgery. The relationship of BMI and infectious complications is also not very clear. Some studies reported that high BMI had a risk for DSWI as well as vein harvest infection (VHI) after CABG surgery. This risk of infection with high BMI was due to higher DM occurrence and because of low oxygen supply at the wound site.8

Human serum albumin (HAS) is used to treat several diseases, such as hypovolaemia, surgical blood-loss, burns and shocks, cardiopulmonary bypass, respiratory distress syndrome (RDS), nutrition support and hypo-albuminaemia.9 Several studies indicate low serum album as a strong predictor of mortality in different surgical procedures, including prostate, gynaecological and cardiac ones. This high rate of mortality is the relation of low albumin with inflammation, which activates response proteins that increases the permeability of vessels for protein and reduces the synthesis of protein in the liver.10 It is clearly indicated that albumin levels <30g/L prior to surgery is an independent risk factor for sternal wound infection and mortality.6 Likewise, pre-operative malnutrition is also a risk factor for both wound infection and mortality.11 Considering malnutrition as a co-morbid factor for post-operative outcomes, nutritional assessment and provision of dietary counselling plays an important role in overcoming malnutrition. In line with literature,12 the current study was planned to investigate the relationship between nutritional status of CABG surgery patients based on BMI, biochemical analysis and diet history with wound healing and post-operative complications in adult patients.


Patients and Methods


The hospital-based prospective study was conducted at the Armed Forces Institute of Cardiology / National Institute of Heart Disease (AFIC / NIHD), Rawalpindi, Pakistan, from October 2017 to March 2018. Using homogenous purposive sampling,12 sample size was calculated with the help of following formula:

P = prevalence of disease 30%

Z = Level of confidence 95%

d = error 5%

                     n= Z2 P (1-P) /d2

                     = (1.96)2 x (0.3) x (1-0.3) / (0.05)2

                     = 3.84 x 0.3 x 0.7 / 0.0025

                     = 0.8064 / 0.0025

                     = 322

Patients of either gender aged 30-70 years admitted in hospital for CABG surgery were included. Data of those who died during or after surgery were excluded. All patients were followed up from admission in hospital (pre-operative) to one week after discharge from hospital for sternal wound healing assessment. The study was approved by hospital Institutional Ethical Review board letter no. 26/7/ R & D 2017/29 and written informed consent was taken from all patients/family enrolled in the study.

Demographic and biochemical data was noted through detailed history-taking and from the hospital record during pre- and post-operative assessment for CABG surgery.

Pre-operative nutritional status of patients was assessed by anthropometric, biochemical, clinical and dietary (ABCD) method.13 According to Asian BMI, the patients were divided into underweight (BMI<18.5), normal (BMI=18.5-22.9), overweight (BMI=23-24.9), moderate obesity (BMI=25-30) and severe obesity (BMI>35) groups14. Biochemical assessment comprised serum albumin (34-50g/L), random blood glucose (RBG) (<200mg/dL), sodium (135-145mmol/L), potassium (3.2-5.2mmol/L), and haemoglobin (12-15g/dL). After clinical examination, dietary history was taken using the semi-quantitative food frequency questionnaire (FFQ).15 The amount, frequency and type of the food consumed was recorded by using household utensils, like cup, glass, spoon, plate, as standard measures for portion size. Frequency of foods was also measured as daily and weekly. Patients were asked about any change in dietary pattern due to the existing diseased condition and were requested to report the information about dietary intake during the preceding 4 weeks according to routine dietary habits. Complete and accurately-filled FFQs were used to compose results by using a food database.16,17

All the patients who underwent CABG surgery were followed up for one week after discharge from hospital. The patients were asked to report to the cardiac surgeon after one week of discharge for sternal wound healing assessment. The scoring system used to assess the wound healing process was Additional treatment, Serous discharge, Erythema, Purulent exudate, Separation of deep tissues, Isolation of bacteria, and Stay as inpatient prolonged over 14 days (ASEPSIS).18 Wound healing was categorised satisfactory healing, disturbance of healing, minor wound infection, moderate wound infection and severe wound infection. Post-operative parameters included stay in intensive care unit (ICU), length of stay in hospital, gastrointestinal (GI) infection (diarrhoea) and chest infection (cough and presence of sputum). Energy intake per kilogram of body weight of the subjects was compared with the recommendations of the American dietary guidelines matched for age, gender and physical activity level.19

Data was analysed using SPSS 21. Data was presented as frequencies and percentages or mean and standard deviation, as appropriate. Pearson's correlation was used to find out the association between independent and dependent categorical variables. One-way analysis of variance (ANOVA) was used to find out the effect of nutritional status on wound healing. Probability level of p<0.05 was considered statistically significant.




Of the 330 patients, 16(4.8%) died within hospital. The final sample, as such, comprised 314(95.2%) patients; 273(86.9%) males and 42(13.1%) females, with an overall mean age of 56.6±8.3 years. The mean body mass index was 26.9±4.3kg/m2 (Figure).



Mean serum albumin was 33.8±7.1 and 180(57.3%) subjects had low albumin levels <35 g/L pre-operatively. According to pre-operative analysis of risk factors, 197(62.7%) patients were in normoglycaemic state, 257(81.1%) were non-hypertensive, 70(22.3%) were current smokers and 244(77.7%) were non-smokers. The presence of DM and HTN was non-significant (p=0.06 and p=0.43, respectively)

BMI and sternal wound healing showed non-significant equation (p>0.05). Significant association was found between blood glucose and wound healing (p<0.05). Serum albumin showed significant association (p=0.03) with wound healing. No association was found of serum sodium, potassium and haemoglobin with wound healing (Table-1).



Overall, 133(42.1%) patients showed clinical signs and symptoms; 80(25.4%) with pale eyes, 25(7.9%) with leukonychia, 18(5.7%) with dry hair, and 10(3.1%) with dry skin.

All food groups and nutrients taken by the subjects showed non-significant relationship with wound healing (p>0.05) (Table-2).






Malnutrition, according to the European Society of Clinical Nutrition and Metabolism (ESPEN), is under or over-nutrition that changes body composition and alters cell mass that leads to impaired physical and mental health and poor outcomes after diseased condition.4 Gucu et al. concluded that malnutrition is associated with failure/delayed wound healing, change in inflammatory and immunity responses, life quality, hospital stay and morality.20 The current study found that BMI was not associated with sternal wound infections. Satisfactory healing was observed more in overweight patients compared to normal-weight patients. Gillis and Carli suggested similar results.21 A prospective study on CABG patients reported no risk of mortality and post-operative complications in overweight and obese populations.22 Across-sectional study on 101 CABG surgery patients reported no association of obesity and post-operative complications.23

While obesity is a risk factor for post-operative complications, it remains a protective factor against post-surgical excessive bleeding in CABG surgery, as in obese patients, large abdominal pressure and mediastinal fat compress the bleeding site by increasing intra-thoracic pressure.24 A study reported low risk of mortality after cardiac surgery in overweight people. While malnutrition (obesity) with diabetes is directly associated with sepsis, peri-operative myocardial infarction (MI) and increased hospital stay in cardiac surgery patients.6 Similar results were reported by another study.25 In the present study, diabetic patients showed satisfactory wound healing perhaps due to good diabetic control in pre-, peri- and post-operative phases. A study reported significant reduction in sternal wound infection with improved glycaemic control in the post-operative phase. In a study, 66% reduced risk of wound infection was reported by using intravenous (IV) insulin dosage to keep blood glucose <200mg/d.26

The present study revealed that albumin can be the indicator of pre-operative nutritional status and had significant association with poor wound healing. Patients with albumin <35mg/dl reported disturbance of wound healing in the present study in line with literature.4 Hypo-albuminaemia was reported as an independent predictor of increased post-operative nosocomial infections, organ dysfunction and morbidity and mortality after cardiac surgery.6 It is very clear that low body protein is related to anaemia, muscle weakness, poor wound healing, increased infection rate and impaired GI mobility.27

Generally, nutritional status is determined by various methods, including anthropometric measures, serum albumin, and total lymphocyte count (TLC). Different nutrition screening methods and tools based on subjective data analysis are used while other tools are based on clinical and biochemical data. It is important to use both subjective and objective data to assess the nutritional status to get more precise and accurate information.28 Nutritional data showed statistically non-significant relationship, but there existed a clinical significance of macro and micronutrients with sternal wound healing. Similar results have been reported in literature on this count.28,29 It was stated that energy is essential for nitrogen synthesis, anabolism and wound healing. For collagen formation glucose is considered a major fuel compared to fat and protein.29 On the basis of food group calculation, the current study population reported high intake of carbohydrate. A study concluded that in daily caloric intake, more calories come from cereal group followed by refined sugars.29 It was reported that high-carbohydrate diet and insulin resistance (IR) contributes to the lipogenesis (metabolic formation of complex fats) and induce the synthesis of pro-inflammatory saturated fatty acids.17 In the current study, the intake of saturated fatty acid and trans-fat was found to be very high. Trans-fats should be mentioned on food labels and provision of trans-fat-free products should reduce its intake to achieve the recommended level.30In the wound healing process, the role of fats is not very clear, but some studies reported anti-inflammatory effect of w-3 fatty acids.6

In the present study, cholesterol intake was high in 57% participants. Some studies have reported the association between increased dietary cholesterol with increased CADs.31 Vegetables and fruits provide several micronutrients. Like flavonoids, carotenoids, and vitamin C, that help in the immune functions and ultimately improve physiological functions, including wound healing.32 It is an established fact that high intake of fruits and vegetables prevent CVDs and improve overall health. Very low intake of vegetables and fruits has been found to be harmful,33 but the current study showed very low intake of fruits and vegetables. A meta-analysis reported that 3-5 servings of fruits and vegetables every day reduces the chances of chronic heart disease by 17%.17 Another study supported the fact that antioxidant vitamins A, E and C show anti-inflammatory effects.34

Dietary fibre intake was reported low in the present study. Some studies have shown the association between high fibre intake and lower levels of low-density lipoprotein (LDL) and glucose, but fibre supplements are not associated with reduction in heart disease.35 Some data suggest that high fibre diet have effect on high glycaemic load.17

It was very clear from the above discussion that pre-operative nutritional status plays an important role after CABG surgery.5 Another study reported that nutrition correction might be effective in primary and secondary prevention, and goals should be set for healthy eating patterns that automatically support health.17




Low levels of albumin and poor blood glucose control before surgery was found to be associated with delayed wound healing. Nutritional intervention prior to CABG surgery may lead to reduced risk and complications during/after surgery. Obese patients who underwent CABG surgery had satisfactory wound healing. Obesity did not relate to poor wound healing, but appears to be a risk factor for post-operative wound healing. Besides, pre-operative hypo-albuminaemia was found to be a good indicator of poor nutritional status and had direct association with poor wound healing.


Disclaimer: None.

Conflict of Interest: None.

Source of Funding: None.




1.       Amini M, Zayeri F, Salehi M. Trend analysis of cardiovascular disease mortality, incidence, and mortality-to-incidence ratio: results from global burden of disease study 2017. BMC Public Health 2021; 21: 401.

2.       Benjamin EJ, Blaha MJ, Chiuve SE, Cushman M, Das SR, Deo R, et al. American Heart Association Statistics Committee and Stroke Statistics Subcommittee. Heart Disease and Stroke Statistics-2017 Update: A Report From the American Heart Association. Circulation 2017; 135: e146-e603.

3.       Melly L, Torregrossa G, Lee T, Jansens JL, Puskas JD. Fifty years of coronary artery bypass grafting. J Thorac Dis 2018; 10: 1960-7.

4.       Keskin M, ?pek G, Alda? M, Altay S, Hay?ro?lu M?, Börklü EB, et al. Effect of nutritional status on mortality in patients undergoing coronary artery bypass grafting. Nutrition 2018; 48: 82-6.

5.       Kulik A, Ruel M, Jneid H, Ferguson TB, Hiratzka LF, Ikonomidis JS, et al. American Heart Association Council on Cardiovascular Surgery and Anesthesia. Secondary prevention after coronary artery bypass graft surgery: a scientific statement from the American Heart Association. Circulation 2015; 131: 927-64.

6.       Lopez-Delgado JC, Muñoz-del Rio G, Flordelís-Lasierra JL, Putzu A. Nutrition in Adult Cardiac Surgery: Preoperative Evaluation, Management in the Postoperative period, and Clinical Implications for Outcomes. J Cardiothoracic Vasc Anesth 2019; 33: 3143-62.

7.       Gao M, Sun J, Young N, Boyd D, Atkins Z, Li Z, et al. Impact of body Mass Index on Outcomes in Cardiac Surgery. J Cardiothoracic Vasc Anesth 2016; 30: 1308-16.

8.       Devarajan J, Vydyanathan A, You J, Xu M, Sessler DI, Sabik JF, et al. The association between body mass index and outcome after coronary artery bypass grafting operations. Eur J Cardiothorac Surg 2016; 50: 344-9.

9.       Fanali G, di Masi A, Trezza V, Marino M, Fasano M, Ascenzi P. Human serum albumin: from bench to bedside. Mol Aspects Med 2012; 33: 209-90.

10.     Meyer CP, Rios-Diaz AJ, Dalela D, Ravi P, Sood A, Hanske J, et al. The association of hypoalbuminemia with early perioperative outcomes- A comprehensive assessment across 16 major procedures. Am J Surg 2017; 214: 871-83.

11.     Lazar HL, Salm TV, Engelman R, Orgill D, Gordon S. Prevention and management of sternal wound infections. J Thorac Cardiovasc Surg 2016; 152: 962-72.

12.     Pourhoseingholi MA, Vahedi M, Rahimzadeh M. Sample size calculation in medical studies. Gastroenterol Hepatol Bed Bench 2013; 6: 14-7.

13.     Sezen Ö, Cevik B. The Impact of Preoperative Nutritional Status of Elderly Patients on the Postoperative Outcome: Comparison of Two Nutritional Assessment Tests and Biochemical Tools. Cyprus J Med Sci 2020; 5: 333-9.

14.     WHO Asian-BMI classification World Health Organization (WHO). International Association for the Study of Obesity (IASO) and International Obesity Task Force (IOTF). The Asia-Pacific Perspective: Redefining Obesity and its Treatment. Geneva: World Health Organization; 2000. p. 378-420.

15.     Farhangi MA, Najafi M. Dietary inflammatory index: a potent association with cardiovascular risk factors among patients candidate for coronary artery bypass grafting (CABG) surgery. Nutr J 2018; 17: 20.

16.     Lowe NM, Ellahi B, Bano Q, Bangash SA, Mitra SR, Zaman M. Dietary calcium intake, vitamin D status, and bone health in postmenopausal women in rural Pakistan. J Health Popul Nutr 2011; 29: 465-70.

17.     Ruiz-Núnez B, van den Hurk G, de Vries J, Mariani MA, de Jongste MJ, Dijck-Brouwer DA, et al. Patients undergoing elective coronary artery bypass grafting exhibit poor pre-operative intakes of fruit, vegetables, dietary fibre, fish and vitamin D. Br J Nutr 2015; 113: 1466-76.

18.     Copanitsanou P, Kechagias VA, Grivas TB, Wilson P. Use of ASEPSIS scoring method for the assessment of surgical wound infections in a Greek orthopaedic department. Int J Orthop Trauma Nurs 2018; 30: 3-7.

19.     U.S. Department of Health and Human Services and U.S. Department of Agriculture. 2015 - 2020 Dietary Guidelines for Americans. 8th ed. [Online] December 2015 [Cited 2021 June 15]. Available at

20.     Gucu A, Ozluk OA, Sunbul SA, Kahraman N, Demir D, Seker IB, et al. Obvious but Always Ignored: Nutritional Status Affects Cardiac Surgery Mortality. [Online] [Cited 2021 June 15]. Available from: URL:

21.     Gillis C, Carli F. Promoting Perioperative Metabolic and Nutritional Care. Anesthesiology 2015; 123: 1455-72.

22.     Boehm J, Lange R, Krane M. Underweight or Obesity Paradox: Do BMI Classifications Predict Long-Term Survival after Cardiac Surgery? The Thoracic and Cardiovascular Surgeon 2019; 67: DGTHG-V127. [not found]

23.     Costa VE, Ferolla SM, Reis TO, Rabello RR, Rocha EA, Couto CM, et al. Impact of body mass index on outcome in patients undergoing coronary artery bypass grafting and/or valve replacement surgery. Rev Bras Cir Cardiovas 2015; 30: 335-42.

24.     Allama A, Ibrahim I, Abdallah A, Ashraf S, Youhana A, Kumar P, et al. Effect of body mass index on early clinical outcomes after cardiac surgery. Asian Cardiovasc Thorac Ann 2014; 22: 667-73.

25.     Thourani VH, Keeling WB, Kilgo PD, Puskas JD, Lattouf OM, Chen EP, et al. The impact of body mass index on morbidity and short- and long-term mortality in cardiac valvular surgery. J Thorac Cardiovasc Surg 2011; 142: 1052-61.

26.     Zhou K, Lansang MC. Diabetes Mellitus and Infections. [Updated 2021 Mar 16]. In: Feingold KR, Anawalt B, Boyce A, et al., editors. Endotext. South Dartmouth (MA):, Inc.; 2000. [Online] [Cited 2021 May 20]. Available from: URL:

27.     Moon MS, Kim SS, Lee SY, Jeon DJ, Yoon MG, Kim SS, et al. Preoperative nutritional status of the surgical patients in Jeju. Clin Orthop Surg 2014; 6: 350-7.

28.     Kunimura A, Ishii H, Uetani T, Aoki T, Harada K, Hirayama K, et al. Impact of nutritional assessment and body mass index on cardiovascular outcomes in patients with Stable Coronary artery disease. Int J Cardiol 2017; 230: 653-58.

29.     Stechmiller JK. Understanding the role of nutrition and wound healing. Nutr Clin Pract 2010; 25: 61-8.

30.     Sacks FM, Lichtenstein AH, Wu JH, Appel LJ, Creager MA, Kris-Etherton PM, American Heart Association, et al. Dietary Fats and Cardiovascular Disease: A Presidential Advisory From the American Heart Association. Circulation 2017; 136: e1-e23.

31.    Carson JAS, Lichtenstein AH, Anderson CAM, Appel LJ, Kris- Etherton PM, et al; American Heart Association Nutrition Committee of the Council on Lifestyle and Cardiometabolic Health; Council on Arteriosclerosis, Thrombosis and Vascular Biology; Council on Cardiovascular and Stroke Nursing; Council on Clinical Cardiology; Council on Peripheral Vascular Disease; and Stroke Council. Dietary Cholesterol and Cardiovascular Risk: A Science Advisory From the American Heart Association. Circulation 2020; 141: e39-e53.

32.    Rosenberg M. Correlations Between Fruit and Vegetable Consumption and Socioeconomic Factors Among Patients with Chronic Venous Leg Ulcers: The Ohio State University; 2016.

33.    Eustachio Colombo P, Milner J, Scheelbeek PFD, Taylor A, Parlesak A, Kastner T, et al. Pathways to" 5-a-day": modeling the health impacts and environmental footprints of meeting the target for fruit and vegetable intake in the United Kingdom. Am J Clin Nutr2021; 114: 530-39.

34.    Wernick B, Nahirniak P, Stawicki SP. Impaired Wound Healing. 2021 Sep 6. In: StatPearls [Online]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-. PMID: 29489281.

35.    Krauss RM, Eckel RH, Howard B, Appel LJ, Daniels SR, Deckelbaum RJ, et al. AHA Dietary Guidelines. Revision 2000: A Statement for Healthcare professionals from the Nutrition Committee of the American Heart Association 2000; 102: 2284-99.

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