July 2021, Volume 71, Issue 7

Original Article

Distinctive morphometric measurement of frontal sinuses among the female ethnic populations living in Karachi

Quratulain Javaid  ( Department of Anatomy, Bahria University Medical and Dental College, Karachi, Pakistan. )
Ambreen Usmani  ( Department of Anatomy, Bahria University Medical and Dental College, Karachi, Pakistan. )

Abstract

Objective: To determine uniqueness of frontal air sinuses in female ethnic populations living in Karachi.

Methods: The cross-sectional descriptive study was conducted at the Radiology Department of Jinnah Postgraduate Medical Centre, Karachi, from November 2018 to April 2019, and comprised adult females who were divided into four equal ethnic groups. Frontal sinus X-ray radiography was done using the Water's view. Width, height and area of the right and left frontal sinuses were measured. The radiographs were saved in photograph format and measurements were done using RadiAnt DICOM software. Measurements of height, width and area were noted. Data was analysed using SPSS 23.

Results: There were 108 females; 27(25%) in each of the four groups. The overall mean age was 35.14±8.68 years. Variability in terms of width, height and area were noted in both right and left frontal air sinuses (p<0.001).

Conclusion: The width, height and area of right and left frontal sinuses showed variability among female ethnic groups living in Karachi.

Keywords: Anatomy, Ethnicity, Frontal sinus, Female, Morphometry. (JPMA 71: 1725; 2021)

DOI: https://doi.org/10.47391/JPMA.866

 

Introduction

 

Every person in a society is known to others by some specific identity. The identification is important for various social and legal reasons. The challenge of identification of the unknown bodies becomes enormous once there are catastrophes as the bodies' configuration becomes damaged and devastated.1

There are three commonly used methods for the identification of unknown victims; soft tissue imaging, analysis of deoxyribonucleic acid (DNA), and the radiography of bony structures. The soft tissue imaging technique of finger printing employs the use of distinctive anatomical patterns in the fingers.1,2 The DNA interpretation of the sample can be achieved by the help of the soft as well as hard tissues. There are prerequisites for carrying out DNA analysis. The quality of the tissue has to be of certain type which does not affect the process of polymerase chain reaction (PCR). Disasters that result in high temperatures cause the chemical composition of the DNA to be changed and make the method inappropriate and unauthentic.3 Mass explosions and catastrophes often result in soft tissue loss. As a consequence, the soft tissue imaging and DNA analysis method become unsuitable. In circumstances of calamities that result in decomposition and even putrefaction of the bodies, the only possible option left is the use of radiography. Compared to other procedures, radiography is considered to be simple and cost-effective.4

The proof of identity is dependent on certain essential factors. Firstly, the structure should be of unique anatomical framework. Secondly, the structure should have the ability to remain constant and should not change over a period of time. Various softwares are available for record-keeping of personal details. Antemortem record-keeping serves as a key stone in identification of individuals with authenticity.5,6 The features available in postmortems are required to be comparable to antemortem data. Many methods can match the postmortem frontal sinus radiographs with that of the antemortem images. These include matching, superimposition and digital analysis methods.5,7,8

There are many bones which have peculiar features as well as strength that can make them stay undamaged and intact even in mass causalities. Skull is used when the victims' bodies suffer from defacement and vandalism as a result of mutilation. Most commonly, frontal sinuses are used for the purpose of identification.1 Frontal sinuses are two in number and are located within the frontal bone. Other than the idiosyncratic features of frontal sinuses, they have the ability to remain intact even after fatal calamities. Frontal sinuses have very strong walls and are located in a safe location within the convexity of the frontal bone. The characteristic convex shape of the frontal sinus shields from destruction.1,9 A study revealed distinctive anatomical features of frontal sinuses among the research participants.10

In Pakistan, disasters are on the rise. A report by the Global Terrorism Data (GTD) and Pakistan Emergency Database (EM-DAT) has mentioned that from 2003 to 2017, there were 45 natural and 289 human-oriented tragic incidents.11 Another report said the number of incidents involving tragic violence and mass explosions were on the rise from 2011 to 2015.12

World over, frontal sinus anatomy is being used as an aid in personal identification. In Pakistan, no research has been done so far related to the use of frontal sinus anatomy for identification. The current study was planned to document the morphometric dimensions and variations among the female ethnic groups living in Karachi.

 

Subjects and Methods

 

The cross-sectional descriptive study was conducted at the Jinnah Postgraduate Medical Centre (JPMC), Karachi, from November 2018 to April 2019. After approval from the institutional ethics review committee (ERC) as well from the ERC of Bahria University Medical and Dental College, Karachi, the sample size was estimated using OpenEpi version 3 calculator.13 The sample size was based on population prevalence 50%, margin of error 5% and confidence interval (CI) 95%. The sample was raised using convenience sampling technique from among females aged 20-50 years who had no paranasal sinus disease. This particular age range was selected because frontal sinus is developed completely till the age of 20 and age-related changes occur after age 50.14 Those aged outside the specific age range were excluded and so were pregnant women and those having paranasal diseases or paranasal sinus surgeries. After taking informed consent from each participant, the sample was divided into Sindhi, Pathan, Punjabi and Urdu-speaking ethnic groups.

Demographic data of the research subjects was noted in a proforma. X-ray radiography (STEPHNEX machine) was used to measure dimensions of the frontal air sinuses. The view used was Water's view, also known as the occipito-mental view. Each subject was asked to stand erect while facing the detector (Figure-1).

The chin was raised so that the mento-mandibular line became perpendicular to the receptor. The X-ray beam was placed horizontal at a point that was one inch above the region of the external occipital protuberance. X-ray beam was placed perpendicular to the radiographic plate. The X-ray beam was at an angle of 45 degrees to the orbito-meatal line. Each radiograph measured 10×12cm in size. The distance was 36cm from the source to film using an exposure of 125KVP at 630mA. The radiographs were saved in high-resolution Joint Photographic Experts Group (JPEG) file and were measured by the help of RadiAnt DICOM Viewer software. The measurements were done in accordance with the guidelines set in literature.8 Height, width and area were noted in the proforma. Each proforma was assigned a code number.  The linear measurements of the width and height were expressed in cm. The area unit used was cm2.

For the measurement of the two frontal air sinuses, baseline A was initially drawn on the software at the upper region of the two orbits. The uppermost point of the right frontal air sinus was marked as B and then the right frontal sinus height C was measured from the marked point B to the point A by drawing a line. The uppermost region of the left frontal air sinus was labelled D and then the left height was measured between points A and D by drawing line E. The lateral most limit of the right sinus was marked as F and then the right frontal sinus width was measured by making line G between inter-frontal sinus septum and line F. The most lateral limit of the left frontal air sinus was marked as H and then between the inter-frontal sinus septum and line H, the left frontal sinus width I was measured. The right area was calculated by multiplying the right height with that of the left frontal sinus width, and the left frontal sinus area was calculated as a multiple of left-sided width and height (Figure-2).

Data was analysed using SPSS 23. Distribution of data was checked using histogram and it was found to be normally distributed. Continuous data was represented as mean ± standard deviation. One-way analysis of variance (ANOVA_ was used to compare the parameters among the ethnic groups. P<0.05 was considered significant, and p<0.001 it was regarded as highly significant.

 

Results

 

There were 108 females; 27(25%) in each of the four groups. The overall mean age was 35.14±8.68 years. Highly significant variability was found in terms of width, height and area in both right and left frontal air sinuses (p<0.001). In all measurements, the highest mean readings were recorded for the Pathan group and the lowest in the Sindhi group (Table).

 

Discussion

 

The anatomical configuration of the frontal sinuses has diverse characteristics in every individual. The unique appearance of the frontal air sinuses is due to the variable developmental process. The final size of the frontal sinuses are dependent on the amount of spongy bone that gets absorbed in between the inner and the outer layers of the frontal bone.8 The present study documented variability in the morphological pattern of the frontal sinuses. Similar findings in terms of the anatomical distinctive anatomy were also documented in a study conducted in Nigeria.13 Corresponding outcomes in terms of anatomic variances of frontal air sinuses were also observed in Karnataka and Manipal states of India.10,14

The current study included the research participants aged 20-50 years. A study conducted on the Indian subjects included subjects within the same age range.14 A study documented that the development of the frontal sinus is finished by the age of 14 years.15 Another study recognized that the frontal sinus growth ends when the person reaches the age of puberty.16 A radiographic study in India documented that the growth of the frontal sinuses showed completion when the individual reaches age 20.17

The present study observed that there existed variability in terms of dimensions of the frontal air sinuses among different ethnic group members living in Karachi. The highest dimensions were observed for Pathan subjects, followed by Punjabis, Urdu-speaking and Sindhi groups. Analogous variations were reported in studies conducted in different ethnic groups living in India.18,19 Unique anatomical features were also observed in relation to the frontal sinus height in studies conducted in Turkey.20,21 The variability of frontal air sinuses dimensions can be linked to the fact that every person's developmental anatomy is different. The quantity of diploe in between the two layers of the compact bone is variable. The amount of diploe represents bone mass that remains after absorption of bone. Since the reabsorption rate is inconstant, all the individuals develop certain characteristic features of frontal sinuses that are unique and distinguishing.9

In the current study, variability in terms of right frontal sinus width was noted among the participants. When the mean left frontal widths were compared, exclusive features that were characteristic for each individual were noted. On both sides, the highest values were calculated for Pathan group and the lowest ones for Sindhi group. Variances in terms of mean width was also observed in studies conducted in India.14,19 A study in India reported the association of metopism with the modification of structural anatomy of frontal sinuses. In all such persons, it was found that metopism hinders the developmental growth and expansion of the frontal air sinuses. As a result, the individuals develop asymmetrical frontal sinuses leading to unique frontal sinus anatomy.22 The distinction in anatomical features of widths in the present study could be linked to the variable pattern of pneumatisation in every individual. The creation of characteristic and unique air-filled spaces within the frontal bone is associated with differences in the size which is specific to each person.23

In the present study, dimensional variability in terms of the frontal air sinus area was also noted. Differences were also noted on the left side among the ethnic groups. On both left and right sides, the greatest values were recorded for Pathan group, followed by Punjabi, Urdu-speaking and Sindhi groups. Variances in terms of mean area were also observed in studies conducted in India.10,18 A study documented that the variable anatomical details could be due to the presence or absence of metopic sutures in the skull. It was mentioned that persons in whom metopic sutures persist after the normal age of closure are associated with small frontal sinuses and in those in which it is closed at the normal age of closure, the size of the frontal sinuses becomes bigger.24 There are many factors which contribute to the development and growth of the frontal air sinuses. A study in Thailand observed the effect of factors that influence the development of paranasal sinuses in identical and non-identical twins, and reported that there can be genetic influence on the expansion, extension and the overall development of the frontal sinuses. Besides, the variation in the right and left frontal air sinuses among the individuals could also be due to the presence and existence of inconstant environmental factors.25

The current study has limitations, as convenience sampling was used instead of probability sampling. Besides, it was conducted at a single centre in Karachi, and therefore, the results cannot be generalised.

There is a need to continue studying the phenomenon at multiple centres in order to include all the other ethnicities and to document the variations on a larger scale.

The frontal air sinus anatomy can be easily figured by observing Water's view radiographs. This radiographic view is commonly prescribed by doctors to rule out paranasal sinuses pathology. In our setup, there is no software available to save the radiographs of patients coming to the hospitals. If we develop a specialised system that can save Water's view radiographic records of the patients, it can later serve as an aid in the identification of charred and the severely-mutilated bodies after comparing the antemortem radiographs with postmortem radiographs.

Despite the limitations, the findings can be used for future studies in Pakistan. The current study, to our knowledge, is the first of its type in the country. This can be very helpful in the fields of forensic sciences and anthropology. The cost-effective procedure which was used to measure the dimensions of frontal air sinuses can be employed nationwide in determining the uniqueness of frontal sinuses.

 

Conclusion

 

Width, height and area displayed unique anatomical configuration of frontal air sinuses on both right and left sides among female ethnic groups living in Karachi.

 

Disclaimer: The text is based on an MPhil (Anatomy) thesis.

Conflict of Interest: None.

Source of Funding: None.

 

References

 

1.      Javaid Q, Usmani A. Anthropological Significance of Sexual Dimorphism and the Unique Structural Anatomy of the Frontal Sinuses: Review of the available literature. J Pak Med Assoc. 2020; 70:713-18.

2.      Dhall JK, Kapoor AK. Fingerprint ridge density as a potential forensic anthropological tool for sex identification. J Forensic Sci. 2016; 61:424-29.

3.      Mienkerd S, Nettakul A, Waiyawuth W. The using of massively parallel sequencing of mitochondrial DNA to assist the missing person identification: Human remains in the wild. Forensic Sci Int. 2019; 7:716-7.

4.      Javaid Q, Usmani A. Variability of Anatomical Features of Frontal Sinuses among the Male Ethnic Groups Residing in Karachi. Professional Med J. 2020; 27:1259-66.

5.      Ubelaker DH, Shamlou A, Kunkle A. Contributions of forensic anthropology to positive scientific identification: a critical Review. Forensic Sci Res. 2019; 4:45-50.

6.      Javaid Q, Usmani A. Anatomical Variations of Frontal Sinuses among the Male and Female Genders Living in Karachi. JBUMDC. 2020; 10:44-8.

7.      Vyas T. Radiographic determination: An upcoming aid in forensic radiology. J Int Clin Dent Res Organ. 2019; 11:71-5.

8.      Ribeiro FDA. Standardized measurements of radiographic films of the frontal sinuses: An aid to ENT. Ear Nose Throat J. 2000; 79:26-8.

9.      Standring S. Gray's anatomy: the anatomical basis of clinical practice. 45th ed. Elsevier Health Sciences, 2015; pp-556-7.

10.    Soman BA, Sujatha GP, Lingappa A. Morphometric evaluation of the frontal sinus in relation to age and gender in subjects residing in Davangere, Karnataka. J Forensic Dent Sci. 2016; 8:57-61.

11.    Najam NUS, Mehmood AM. The economic cost of terrorism and natural disasters: A deeper analysis of the financial markets of Pakistan. [Online] 2019 [Cited 2019 04 11]. Available from: URL: https://mpra.ub.uni-muenchen.de/92278/

12.    Choudhary A, Lemos R, Van Reenen J. Management in Pakistan: Performance and conflict. IGC 2017; 89451:1-46.

13.    Eboh DE, Ogbeide OU, Ivwighren T. Radiographic anthropometric study of frontal sinus for sex determination in Benin city, South-South Nigeria. J Forensic Dent Sci. 2017; 9:31-5.

14.    Pradhan A, Dakhar W, Shajan M. Measurement of different dimension of maxillary and frontal sinus through computed tomography. Online J Health Allied Sci.2017; 16:1-3.

15.    Moore K, Ross A. Frontal sinus development and juvenile age estimation. Anat Rec (Hoboken). 2017; 300:1609-17.

16.    Valverde Y, Watanabe N, Yamaki M, Saito I. The frontal sinus enlargement as an indicator of growth maturity in class III patients-a pilot study. Int J Med Sci Public Health. 2013; 2:451-5.

17.    Nethan ST, Sinha S, Chandra S. Frontal Sinus Dimensions: An Aid in Gender Determination. Act Scientific Dent Sci. 2018; 2:2-6.

18.    Belaldavar C, Kotrashetti VS, Hallikerimath SR, Kale AD. Assessment of frontal sinus dimensions to determine sexual dimorphism among Indian adults. J Forensic Dent Sci .2014; 6:25-30.

19.    Verma S, Mahima VG, Patil K. Radiomorphometric analysis of frontal sinus for sex determination. JFDS. 2014; 6:177-81.

20.    Buyuk SK, Simsek H, Karaman A. The relationship between frontal sinus morphology and skeletal maturation. Folia Morphol. 2017; 77:503-8.

21.    Tatlisumak E, Asirdizer M, Bora A, Hekimoglu A, Etli Y, Gumus O, et al. The effects of gender and age on forensic personal identification from frontal sinus in a Turkish population. Saudi Med J. 2017; 38:41-7.

22.    Kumar NV, Vijayashanmugam U, Gugapriya TS, Nalinakumari SD, Kumar NV. A roentgen-anatomic study of association between persistent inter-frontal Suture with varied presence of frontal sinus. Indian J Clin Anat Physiol. 2016; 3:510-3.

23.    Yazici D. The effect of frontal sinus pneumatization on anatomic variants of paranasal sinuses. Eur Arch Otorhinolaryngol. 2019; 276:1049-56.

24.    Nikolova S, Toneva D, Georgiev I, Lazarov N. Digital radiomorphometric analysis of the frontal sinus and assessment of the relation between persistent metopic suture and frontal sinus development. Am J Phys. 2018; 165:492-506.

25.    Chaiyasate S, Baron I, Clement P. Analysis of paranasal sinus development and anatomical variations: a CT genetic study in twins. Clin Otolaryngol. 2007; 32:93-7.

 

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